Evaluation of an ELISA to detect rabies antibodies in orally vaccinated foxes and raccoon dogs sampled in the field.
Identifieur interne : 000156 ( Main/Exploration ); précédent : 000155; suivant : 000157Evaluation of an ELISA to detect rabies antibodies in orally vaccinated foxes and raccoon dogs sampled in the field.
Auteurs : M. Wasniewski [France] ; A L Guiot ; J L Schereffer ; L. Tribout ; K. M Har ; F. CliquetSource :
- Journal of virological methods [ 1879-0984 ] ; 2013.
Descripteurs français
English descriptors
- KwdEn :
- MESH :
- chemical , administration & dosage : Rabies Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Rabies Vaccines.
- geographic : Estonia, France.
- methods : Enzyme-Linked Immunosorbent Assay.
- prevention & control : Rabies.
- Administration, Oral, Animals, Foxes, Raccoon Dogs, Sensitivity and Specificity.
Abstract
The assessment of the efficacy of oral vaccination in wildlife is based on detection in the teeth of a biomarker (tetracycline) which is incorporated in the vaccine bait, and the quantification of rabies antibodies. A blocking ELISA was evaluated and compared with the FAVN test and a validated in-house ELISA, using sera from foxes and raccoon dogs collected following oral vaccination campaigns in France and Estonia. Specificity reached 100% in sera from naïve animals. A high concordance (95%) was observed between the BioPro ELISA and the FAVN test, which was similar in sera from red foxes and raccoon dogs. Concordance between the BioPro ELISA and the in-house ELISA reached 96.5% for sera from red foxes. The agreement with tetracycline results was excellent in the fox for both the BioPro ELISA (95.9%) and the FAVN test (91.8%). Concordance was slightly lower in the raccoon dog, with a value of 82.8% for the BioPro ELISA and 78.4% for the FAVN test. Rabies antibodies were detected with the BioPro ELISA in animals vaccinated with different types of vaccines and in highly haemolysed sera. The BioPro ELISA is a valuable test to assess the efficacy of oral vaccination in foxes and raccoon dogs.
DOI: 10.1016/j.jviromet.2012.11.022
PubMed: 23201293
Affiliations:
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Le document en format XML
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<term>Estonia</term>
<term>Foxes</term>
<term>France</term>
<term>Rabies (prevention & control)</term>
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<term>Rabies Vaccines (immunology)</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en">The assessment of the efficacy of oral vaccination in wildlife is based on detection in the teeth of a biomarker (tetracycline) which is incorporated in the vaccine bait, and the quantification of rabies antibodies. A blocking ELISA was evaluated and compared with the FAVN test and a validated in-house ELISA, using sera from foxes and raccoon dogs collected following oral vaccination campaigns in France and Estonia. Specificity reached 100% in sera from naïve animals. A high concordance (95%) was observed between the BioPro ELISA and the FAVN test, which was similar in sera from red foxes and raccoon dogs. Concordance between the BioPro ELISA and the in-house ELISA reached 96.5% for sera from red foxes. The agreement with tetracycline results was excellent in the fox for both the BioPro ELISA (95.9%) and the FAVN test (91.8%). Concordance was slightly lower in the raccoon dog, with a value of 82.8% for the BioPro ELISA and 78.4% for the FAVN test. Rabies antibodies were detected with the BioPro ELISA in animals vaccinated with different types of vaccines and in highly haemolysed sera. The BioPro ELISA is a valuable test to assess the efficacy of oral vaccination in foxes and raccoon dogs.</div>
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